Repression of transcription factor AtWRKY47 confers tolerance to boron toxicity in Arabidopsis thaliana

Repression of transcription factor AtWRKY47 confers tolerance to boron toxicity in Arabidopsis thaliana

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Boron (B) excess gives rise to a serious agricultural problem.In this study, we identified a B toxicity responsive transcription factor AtWRKY47 in Arabidopsis thaliana.The T-DNA insertion mutants Atwrky47 showed enhanced tolerance to B toxicity with better growth parameters under high B conditions compared to wild-type Col-0 plants.Quantitative analysis of AtWRKY47 mRNA abundance indicated that it was down-regulated under B toxicity 3 piece horse wall art conditions.

Fluorescently labeled AtWRKY47 protein was localized in nucleus.In contrast to the phenotype of Atwrky47 mutants, overexpression of AtWRKY47 in Col-0 background resulted in lower biomass, less chlorophyll content, and increased sensitivity to B toxicity.More importantly, the B concentration in shoots was higher in the overexpression lines and lower in the Atwrky47 mutants than in kel instruments soil tester Col-0 plants, respectively.These results demonstrate that AtWRKY47 gene plays a key role in regulating plant tolerance to B toxicity.